Can I add barcodes to a set already in use?

Yes. A locked set is kept as-is and the designer adds new barcodes that respect the minimum distance against it, reporting any pair inside the locked set that is already too close.

Does it check colour balance?

Yes, per cycle, and every violation names the barcodes responsible so the set can be corrected.

Design

Barcode & Index Design

Generate a barcode set whose minimum distance is guaranteed rather than hoped for, inspect every barcode in it, and extend a set you are already committed to. The designer enumerates its own candidate pool, so it needs no input sequences.

Open Barcode & Index Design Open the Barcode & index design sample

Six published FREE-divergence index sets and the paper's own 100-read decoding example: design, audit, prune and demultiplex against reference data

What it does

01Barcode set design with a guaranteed minimum distance
02Per-barcode profile: GC, longest homopolymer run, nearest neighbour and which barcode it collides with
03Set statistics, including distance and nearest-neighbour histograms and per-cycle entropy
04Colour-balance checks per cycle
05QA report where an unsupplied check reports as skipped rather than as passed
06Set comparison, including the minimum distance two sets guarantee when pooled together
07Extension of a locked set, keeping the barcodes already in use
08Export as CSV, TSV, FASTA, JSON or a sample sheet

What it runs on

Design and analysis run on the barcode engine in packages/ports, ported from DNABarcodes and golden-validated against it. The panel runs on @ports/barcode.

Everything runs client-side. Files are parsed in the browser and projects are saved on this machine, so an unpublished sequence is never uploaded.

Questions

Can I add barcodes to a set already in use?: Yes. A locked set is kept as-is and the designer adds new barcodes that respect the minimum distance against it, reporting any pair inside the locked set that is already too close.
Does it check colour balance?: Yes, per cycle, and every violation names the barcodes responsible so the set can be corrected.

Also searched as

DNA barcode design tool · sequencing index design · barcode minimum edit distance · demultiplexing barcode set · illumina index colour balance · UMI barcode generator · extend existing barcode set · sample index design tool · Illumina index set design · Hamming distance barcode set · dual index design · barcode collision checker